Thermal denaturation of subtilisin-SSI complex analyzed by SDS-PAGE.
نویسندگان
چکیده
منابع مشابه
Thermal denaturation of proteins for SDS-PAGE analysis by microwave irradiation.
One of the most useful tools available to researchers is sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) (3). It is a rapid and relatively simple method for resolving proteins and is routinely used to monitor proteins during purification, assess expression levels, estimate molecular weights and characterize multimeric proteins. A number of variations exist, but the method d...
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Methanothermobacter thermautotrophicus is a thermophilic archaeon that produces methane as the end product of its primary metabolism. The biochemistry of methane formation has been extensively studied and is catalyzed by individual enzymes and proteins that are organized in protein complexes. Although much is known of the protein complexes involved in methanogenesis, only limited information is...
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15 صفحه اولTricine – SDS - PAGE Hermann Schägger
Tricine–SDS-PAGE is commonly used to separate proteins in the mass range 1–100 kDa. It is the preferred electrophoretic system for the resolution of proteins smaller than 30 kDa. The concentrations of acrylamide used in the gels are lower than in other electrophoretic systems. These lower concentrations facilitate electroblotting, which is particularly crucial for hydrophobic proteins. Tricine–...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1990
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.54.563